Autoimmune diseases are conditions caused by the immune system's immune response to components of the body itself, resulting in damage. These diseases can affect multiple systems and organs throughout the body, with over a hundred different types. The overall incidence rate of autoimmune diseases globally is approximately 10%. The broad-spectrum autoantibody detection chip covers more than 4000 autoantibody indicators with clinical guidance value, as well as corresponding positive and negative controls. It can be widely used for high-throughput detection of autoantibodies in the body fluids of patients with 25 common autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, and autoimmune hepatitis. This chip is capable of detecting multiple antibody subtypes, including IgM, IgG, IgG1-4, IgA, and IgA1-2.